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2 edition of Gene expression change during teliospore germination in Ustilago maydis. found in the catalog.

Gene expression change during teliospore germination in Ustilago maydis.

Ali Reza Zahiri

Gene expression change during teliospore germination in Ustilago maydis.

by Ali Reza Zahiri

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  • 40 Currently reading

Published .
Written in English


About the Edition

The fungal pathogen Ustilago maydis induces the formation of tumours in maize. Tumour production provides an environment for hyphal differentiation, leading to the formation of thick-walled, diploid teliospores. These spores are a dispersal agent and their germination leads to new rounds of infection. The morphological changes during U. maydis teliospore germination have been described; however, specific molecular events that facilitate this process have not been identified. Through the construction and hybridization of microarrays containing a set of 3918 non-redundant cDNAs we identified genes differentially regulated during germination. Teliospores induced to germinate for 4 and 11 hours were selected for comparison to dormant teliospores. Genes identified as differentially expressed included those representing undescribed molecular events in teliospore germination as well as characterized genes previously shown to be required for teliospore germination. This study represents the first largescale investigation of gene expression change during teliospore germination.

The Physical Object
Pagination79 leaves.
Number of Pages79
ID Numbers
Open LibraryOL19216239M
ISBN 100494071214

  Expression profiles of genes were cross-examined with gene orthologs belonging to expression gene groups obtained during teliospore germination in or those obtained during spore germination in by:   The maize pathogen Ustilago maydis switches from budding to filamentous, dikaryotic growth in response to environmental signals including nutrient status, growth in the host, and the presence of mating pheromones. The filamentous dikaryon is capable of proliferating within host tissue to cause disease symptoms including tumors. The transition Cited by:

  A homologue of the gene encoding the transcription factor Rim (PacC), involved in pH signal transduction in fungi, was identified in the pathogenic basidiomycete Ustilago maydis. The gene (RIM) encodes a protein of amino acid residues, which shows highest similarity to PacC proteins from Fusarium oxysporum and Aspergillus niger. The gene Cited by: Tumor formation in a cob of corn infected with Ustilago picture was taken in a field close to Marburg, Germany.. II. Important tools for experimentation with Ustilago maydis. During the last decades, a large number of molecular techniques has been developed for U. maydis which allow to study gene function in detail. The sporidia can be efficiently transformed using .

The present study was aimed at investigating the role of the PTEN ortholog, ptn1, in Ustilago maydis, the pathogen of maize. U. maydis ptn1 mutant strains where ptn1 gene is deleted or overexpressed were examined for phenotypes associate with Cited by: 1. Teliospore germination and meiosis. The teliospore is a diploid spore, resistant to adverse environmental conditions. It is formed within the tumors following a discrete developmental program during which changes in morphology take place and karyogamy occurs ().Upon germination, a short filament (the promycelium) emerges, into which the diploid nucleus Cited by:


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Gene expression change during teliospore germination in Ustilago maydis by Ali Reza Zahiri Download PDF EPUB FB2

Such spores serve as a dispersal agent for smut and rust fungi, and their germination leads to new rounds of infection. The morphological changes that occur during teliospore germination in U. maydis have been described in detail. However, the specific molecular events that facilitate this process have not been by: Differential gene expression during teliospore germination in Ustilago maydis Article (PDF Available) in Molecular Genetics and Genomics (5) July with Reads.

While there is consensus that a single diploid nucleus is present in mature teliospores and that meiosis ensues when teliospores germinate, there is scant evidence to establish when or how the onset and progression of homologous recombination, the central genetic process underlying meiosis, is coupled to the morphological events of meiosis that manifest during teliospore by: 8.

Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis. Ustilago maydis infection of Zea mays leads to the production of thick-walled diploid teliospores.

The Northern hybridizations of selected identified genes were also used to determine expression profiles in different cell types and during teliospore germination. Hybridization of a putative functional homologue of peroxiredoxin (also called rehydrin), contig T11_, indicates that the transcript is upregulated in the dormant teliospores of U.

maydis (Fig. 3).Cited by: Search term. Advanced Search Citation Search. Login / RegisterCited by:   A central feature of meiosis is the pairing and recombination of homologous chromosomes. Ustilago maydis, a biotrophic fungus that parasitizes maize, has long been utilized as an experimental system for studying recombination, but it has not been clear when in the life cycle meiotic recombination initiates.

maydis forms dormant diploid teliospores as Cited by: 8. Relative expression level of sporulation-specific protein 5 (UeSPR5) gene, polyketide synthase (UePKS) gene, and laccase-1 (UeLAC1) gene in Ustilago esculenta T and M-T strain exposed to heat or cold (A–C) and osmotic (D–F) stress.

Cultures of each strain were grown in YEPS medium for 72 h at 28 °C and then transferred to potato dextrose broth (PDB) incubated at 28 Author: Zheng-Hong Wang, Ning Yan, Xi Luo, Sai-Sai Guo, Shu-Qin Xue, Jiang-Qiong Liu, Jing-Ze Zhang, De-Ping.

In order to study teliospore biology, our laboratory utilizes the model smut fungus Ustilago maydis (U. maydis), which is the causal agent of the disease 'common smut of corn'.

Mature U. maydis teliospores are characterized by growth arrest, reduced cellular metabolism, and low levels of cellular respiration : Lauren A. Ostrowski, Amanda M. Seto, Barry Saville. EXPLORING THE ROLE OF PTN1 IN USTILAGO MAYDIS Lalu M Vijayakrishnapillai Ma Signal transduction is a key aspect of biological life.

PTEN (phosphatase and tensin homolog) is a widely studied signaling protein in mammalian systems, where it acts as a tumor suppressor.

Numerous studies have conclusively demonstrated the anti-cancerousAuthor: Vijayakrishna Pillai, Lalu Murali Krishna. When fungi interact with plants as pathogens or as symbionts, there are often changes in fungal cell morphology and nuclear state.

This study establishes the use of cDNA microarrays to detect gene expression changes in Ustilago maydis cells that differ in structure and nuclear content.

Categorizing differentially expressed genes on the basis of function indicated that U. maydis Cited by:   The maize smut fungus Ustilago maydis is a model organism for elucidating host colonization strategies of biotrophic fungi. Here, we performed an in depth transcriptional profiling of the entire plant-associated development of U.

maydis wild-type strains. In our analysis, we focused on fungal metabolism, nutritional strategies, secreted effectors, and regulatory by:   Differential gene expression during teliospore germination in Ustilago maydis.

Mol Genet Genomics. ;– Mol Genet Genomics. ;– CASCited by: 7. To analyze changes in gene expression during early spore development, we did four pair-wise time point comparisons with a minimum of eight replications for each comparison; 0 h versus 24 h, 6 h versus 24 h, 12 h versus 24 h, and 48 h versus 24 h.

Zheng, Y. et al. The Ustilago maydis Cys2His2-type zinc finger transcription factor Mzr1 regulates fungal gene expression during the biotrophic growth stage. Mol. Microbiol. 68, – ( Cited by:   During maize infection, the fungal pathogen Ustilago maydis undergoes a dimorphic transition from budding, yeast-like cells to a filamentous dikaryon that proliferates in the host.

This transition is regulated by mating and environmental signals. Septation is likely to be important in the growth of the infectious dikaryon because of the need to maintain specific cellular compartments during Cited by:   Gene expression and EST analyses of Ustilago maydis germinating teliospores How these fungi persist between rounds of infection and how they are disseminated between hosts varies with the pathogen.

In bunt, rust, and smut fungi, disease propagation and survival relies on the production of teliospores, resistant diploid cells capable of germination. Understanding the molecular mechanisms of the interaction of Ustilago maydis with maize entails understanding the molecular mechanisms that regulate its life cycle.

This chapter presents an overview of the current knowledge regarding the interaction of Ustilago maydis with maize. First, useful features that have facilitated analysis of the life cycle are described, followed by a Cited by: 2.

Altered timing of sta1 expression interferes with the virulence function of Sta1 in Ustilago maydis. (a) The expression pattern of U. maydis UMAG_ gene during plant infection retrieved from RNA sequencing data (Lanver et al., ). A.C., expression level in axenic culture.

The numbers below the bars indicate the days : Shigeyuki Tanaka, Isabelle Gollin, Nicole Rössel, Regine Kahmann.

The maize smut fungus Ustilago maydis switches from yeast to hyphal growth to infect maize (Zea mays) plants. This switching is promoted by mating of compatible cells and seems to be required for plant penetration. Although many genes distinctively expressed during this dimorphic switch have been identified and shown to be essential for the infection process, Cited by:.

Ustilago maydis is an important fungal pathogen of maize, causing corn smut. It is well adapted to its host and proliferates in living plant tissue without inducing a defence response. The genome.Author Summary The fungus Ustilago maydis is a pathogen of maize which induces tumor formation in the infected tissue.

In these tumors huge amounts of fungal spores develop. As a biotrophic pathogen, U. maydis establishes itself in the plant with the help of a large number of secreted effector proteins.

Many effector proteins are important for virulence because they Cited by:   Ustilago maydis is the basidiomycete fungus responsible for common smut of corn and is a model organism for the study of fungal phytopathogenesis.

To aid in the annotation of the genome sequence of this organism, several expressed sequence tag (EST) libraries were generated from a variety of U. maydis cell types. In addition to utility in the context of gene Cited by: